FREQUENCY DISTRIBUTION OF HOST-RANGE MUTANTS AMONG T2 PHAGE INFECTED BACTERIA

Frequency distribution of host-range mutants among T2 phage infected bacteria.

Phage. A plaque type mutant phage, T2 r tu, was used as a test bacteriophage. Its latent period and burst size, when incubated with Escherichia coli strain B cells in nutrient broth at 37 C, were about 20 min and 50, respectively. Host bacteria. Escherichia coli strain B was used as host. To select for host-range mutants, strain B/2 which is resistant to T2 was used. Techniques. Standard method...

Characterization of Streptococcus thermophilus host range phage mutants.

To investigate phage-host interactions in Streptococcus thermophilus, a phage-resistant derivative (SMQ-301R) was obtained by challenging a Tn917 library of phage-sensitive strain S. thermophilus SMQ-301 with virulent phage DT1. Mutants of phages DT1 and MD2 capable of infecting SMQ-301 and SMQ-301R were isolated at a frequency of 10(-6). Four host range phage mutants were analyzed further and ...

Host range mutants of polyoma virus.

A line of polyoma-transformed mouse cells has been isolated which is fully susceptible to lytic infection by polyoma virus. This line has been used to select virus mutants which have lost most or all of their ability to grow in the untransformed parental line while retaining the ability to grow in the transformed derivative. These virus mutants are also defective in their ability to transform c...

Broadbean wilt virus - identification,host range and distribution in the fars province of iran

Some Properties of DNA from Phage-Infected Bacteria

Replicating T5 or lambda phage DNA has been labeled by adding tritiated thymidine for short periods to cultures of phage-infected Escherichia coli before isolation of intracellular DNA. Two procedures are described for separating T5 replicating DNA from DNA of intracellular phage particles. Both T5 and lambda replicating DNA had the same bouyant density in cesium chloride as DNA from phage part...